Coding

Part:BBa_K317998

Designed by: Daisuke HIRAOKA   Group: iGEM10_Tokyo-NoKoGen   (2010-10-19)

RhlA

Pseudomonas aeruginosa produces rhamnose-containing glycolipid biosurfactants called rhamnolipids. Rhamnolipid is composed of β-D-(β-D-hydroxyalkanoyloxy)alkanoic acids(HAA) derivatized with one- or two-rhamnose sugars.In rhamnolipid biosynthesis, RhlA is required for the formation of the HAA portion of the molecule and RhlB plays a role of the rhamnosyl transferase. When RhlA and RhlB are expressed in E.coli BL21(DE3) strain, rhamnolipid indicating emulsification ability will be produced. This part includes 1 PstI site.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 720
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 720
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 69
    Illegal BamHI site found at 629
    Illegal XhoI site found at 805
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 720
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 720
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 294
    Illegal BsaI.rc site found at 478


[edit]
Categories
Parameters
None